| 摘要: |
| 研究壳聚糖在不同处理条件下，其稳定性和抑菌作用的变化；以大肠杆菌和金黄色葡萄球菌作为实验菌种，主要考察的影响因素包括：壳聚糖浓度（壳聚糖浓度范围0～20 g/L）、温度（冰浴，25 ℃，60 ℃，121 ℃）、金属离子种类（NaCl，MgCl2，FeCl3）和浓度（01 mol/L，02 mol/L，03 mol/L，04 mol/L，05 mol/L）、酸溶剂（HAc、HCl、柠檬酸），滤纸片琼脂扩散法测定各影响因素下，壳聚糖的稳定性及抑菌作用的变化；结果表明：随着壳聚糖浓度的增加，抑菌作用逐渐增强；不同温度下，壳聚糖均能保持较好的稳定性；金属离子种类和浓度对壳聚糖的性能有一定的抑制作用；不同酸溶剂溶解壳聚糖时，其稳定性和抑菌作用存在一定的差异。 |
| 关键词: 壳聚糖 稳定性 抑菌作用 影响因素 |
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| Study on Stability and Antibacterial Activity of Chitosan |
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SONG Jun-mei, ZHANG Xin, GAO Yu-rong1,2
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1.Chaohu University, School of Chemistry and Material Engineering,Anhui Chaohu 238000, China;2.Shanghai Haihong Group Chaohu C-dragon Pharmaceutical Co.,Ltd, Anhui Chaohu 238000,China
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| Abstract: |
| The stability and bacteriostatic effects of chitosan under different treatment conditions were studied. Escherichia coli and Staphylococcus aureus were used as experimental bacteria. The main influencing factors include chitosan concentration(chitosan concentration from 0 to 20 grams per liter), temperature(ice bath, 25℃, 60℃, 121℃), Metal ion species (NaCl, MgCl.2, FeCl.3) and concentration(0.1 mol/L, 0.2 mol/L, 0.3 mol/L, 0.4 mol/L, 0.5 mol/L), acid solvent(HAc, HCl, citric acid). The antibacterial effect of each influencing factor was determined by filter paper agar diffusion. The results show that with the increase of chitosan concentration, the bacteriostatic effect was enhanced, and chitosan has good stability at different temperatures. Metal ion species and concentration have certain inhibiting effect on the properties of chitosan. The chitosan dissolved in different acid solvent, its stability and antibacterial effect were different. |
| Key words: chitosan stability bacteriostasis influence factor |
