| 摘要: |
| 为了更加深入地了解兰科石斛属(Dendrobium Sw.)植物所特有的石斛碱型生物碱，及其代表性成分石斛碱的生物合成途径，对石斛碱型生物碱的结构组成和药理活性，以及石斛碱生物合成等三方面进行了文献综述，并推测了一条以金钗石斛次生代谢产物为可能中间体的石斛碱生物合成通路；发现已报道的石斛碱型生物碱有31个，来源于9种石斛，其药理活性主要在抗肿瘤、抗白内障、降血糖等方面；石斛碱的生物合成部分处于初期研究阶段，仅确定了合成途径为MVA途径以及7个相关基因；最后为石斛碱生物合成研究的深入提出了三个切入点：以法尼基焦磷酸(FPP)为底物通过酶促反应得到可能中间体；将FPP加入金钗石斛组培苗的培养基，促进可能中间体的产生；以石斛碱的累积特点入手，找到中间体最多的点，进行可能中间体的分离。 |
| 关键词: 金钗石斛 生物碱 石斛碱 倍半萜 合成生物学 |
| DOI： |
| 分类号: |
| 基金项目: |
|
| Research Progress of Dendrobine-type Alakaloids and Synthetic Biology of Dendrobine |
|
CHEN Xing-yue，GONG Dao-yong，GUO Shun-xing，LI Biao1,2
|
|
1. Institute of Medicinal Plant Development， Chinese Academy of Medical Sciences，Beijing 100193， China;2. School of Bioengineering, Chongqing University，Chongqing 400045， China
|
| Abstract: |
| In order to have a deeper understanding of the unique dendrobine-type alkaloids of Dendrobium Sw， and the biosynthetic pathway of its representative component dendrobine, in this paper， the structures and pharmacological activities of dendrobine-type alkaloids and the biosynthesis of dendrobine were reviewed， and a pathway of dendrobine biosynthesis， which is mainly secondary metabolites of Dendrobium nobile Lindl. was speculated.The number of reported dendrobium alkaloids is 31， derived from 9 species of Dendrobium Sw， and its pharmacological activities are mainly in anti-tumor， anti-cataract， and hypoglycemic aspects. However， the biosynthesis of dendrobium is in the initial stage of research， and only the MVA pathway and 7 related genes have been identified.Finally， it provides three entry points for the in-depth study of dendrobine biosynthesis research：first， we can use farnesyl pyrophosphate (FPP) as the substrate to obtain possible intermediates by the enzymatic reaction； secondly， we can also add FPP to the culture medium of D. nobileLindl.tissue culture seedlings to promote the possible intermediates； thirdly， we can start with the accumulation characteristics of dendrobine， find the point with the most intermediates and carry out the separation of the possible intermediates. |
| Key words: Dendrobium nobile Lindl alkaloids dendrobine sesquiterpenoid biosynthesis |
