| 摘要: |
| 摘 要:介绍了亲和接头在高通量生产目标蛋白质中的运用,这些亲和接头包括6组氨酸、麦芽糖结合
蛋白、谷胱甘肽- S - 转移酶和硫氧还蛋白,并讨论了高通量生产的蛋白质的溶解和复性。 |
| 关键词: 结构基因组学 亲和接头 融合蛋白 |
| DOI： |
| 分类号:O69 |
| 基金项目: |
|
| Affinity tags in structural genomics research |
|
JIANG Hua i - chun1 , L I Hong2
|
| Abstract: |
| Abstract: This paper introduces app lication of affinity tags to high - throughput p roduction of targeted p roteins, these tags include
hexa - histidine, maltose - binding p rotein, glutathione - S - transferase and thioredoxin and discusses p rotein solubility and refolding
for the p roteins p roduced by high - throughputmethods. |
| Key words: structural genomics affinity tag fusion p rotein |
