摘要: |
摘 要:建立了热水提取、乙醇沉淀、Sevage法除蛋白、有机溶剂分离精制提取黄瓜多糖的方法,并用蒽
酮- 硫酸比色法测定其含量;采用超氧阴离子自由基、羟基自由基、二苯代苦味肼基自由基(DPPH·) 体系,
对黄瓜多糖的抗氧化活性进行了研究,结果表明:黄瓜多糖对DPPH·、·OH and O-
2 ·具有较强的清除能
力,与其浓度呈线性关系。 |
关键词: 黄瓜多糖抗氧化活性研究 |
DOI: |
分类号:O643 |
基金项目:重庆工商大学博士科研启动项目? |
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Study on the antioxidative activites of polysaccharidesfrom cucumis saticus L. |
XU Ping
|
Abstract: |
Abstract: Cucumber polysaccharides (CP) were obtained by hot water extraction, ethanol p recip itation, sevage method removing
p roteins and organic solvent purification. Polysaccharides contentwas determined by anthrone - H2 SO4 colorimeter. Using the superox2
ide radical system , hydroxyl radical system , and system of 2, 2 - Diphenyl - 1 - p icryhydrazyl (DPPH·) , the antioxidation activities
of cucumber polysaccharides is studied . The results showed that CP had strong radical scavenging activity towardsDPPH·, ·OH and
O -
2 ·. The capacity of scavenging free radicals of cucumber polysaccharides showed linear connectionswith the concentration. |
Key words: cucumber polysaccharide free radicals scaveng antioxidant activity |