|
| 摘要: |
| 介绍了X-射线结晶学研究方面的新进展,包括蛋白质基因的克隆和表达载体的选择、蛋白质表达、蛋白质纯化、纯化的蛋白质的质量控制、高通量蛋白质结晶、晶体的收获和储存、同步加速器线束数据收集、自动的晶体固定和观察以及衍射质量分析和数据收集。 |
| 关键词: 结构基因组学,X-射线晶体衍射,自动化,蛋白质 |
| DOI: |
| 分类号:Q781 |
| 基金项目: |
|
| Structural genomics and X-ray crystallography automation |
|
JIANG Huai-chun LI Hong
|
| Abstract: |
| This paper introduces the new progress in X-ray crystallography automation which includes target protein gene clone and expression vector selection,protein expression,protein purification,quality control of purified protein,high-throughput protein crystal |
| Key words: structural genomics,X-ray crystallography,automation,protein |