Ecotourism has become the most potential tourism market as a major trend of modern tourism. As an important destination in China's tourism industry, Guilin’s ecotourism resources are abundant. This paper selects nine representative natural reserves of Guilin, and gives the evaluation on Guilin’s ecotourism resources based on AHP Quantitative Appraisal. The results show that the environmental conditions have become the most attractive factors in the development of Guilin’s ecotourism, and that we should pay more attention to protecting and improving the ecotourism environment and to their ecological recreation while the development of ecotourism should emphasize ecotourism resources conditions and tourism development potential.