This paper reviewed the practice of inland China opening-up, and empirically tested the effect on economic growth through the design of difference in differences model (DID) using panel data covering 31 provinces of China, including municipalities and autonomous regions over the period from 2000 to 2016. The research shows that China Railway Express (CR express) opened a new horizon for inland China openingup practice. From 2011 to 2016, the operation quantity of CR express was undergoing annual average growth of 160%, which effectively improved the transportation conditions for inland China areas to participate in the international trade market. Secondly, the practice of inland opening-up turned inland regions into open frontiers, and finally significantly boosted the economic growth of central and western China. This conclusion can be examined through a series of robustness tests. Thirdly, the fact of inland openingup promoting economic growth was achieved by such two means as improved cost effectiveness and industrial restructuring efforts. As an implication, the success of the practice of inland China opening-up provides a useful reference for other inland countries and regions to develop open economy.