Chongqing’s animation industry has made great progress since Chongqing became the municipality but still lags behind the developed cities at home and abroad.Chongqing should closely combine emerging animation industry with deep nationalities culture of Ba and Yu and pave a road with regional characteristics for animation industry.Through studying the characteristics of Chongqing’s nationalities culture,this paper analyzes the influence and effect of nationalities culture on animation industry development,briefly points out the problems in animation industry development and makes suggestions.