By analyzing the questionnaire survey data of teachers and students using online learning system in the university, reference the traditional learning achievement and knowledge transferring research model, the online learning system revision research model is established and improved, and the model is affected by factors composed of five independent variables which are computer selfefficacy, selflearning ability, ease of use and usefulness, email interaction and social existence. The results of multiple regression analysis show that these five independent variables have different effects on students' online learning effect, and the influence degree is different from that of traditional research models. To improve the efficiency of online learning system, it is necessary to improve the ease of use and usefulness, increase the social existence of online learning system users, and improve the selflearning ability of online learning system users.