The research on the expression effect of traditional architecture elements in an animation scene aims to discuss the methods to use traditional architecture elements in an animation scene. The eyetracing analysis method is used to record eyetracing indexes in each interesting field in an animation scene to try to find their law by data analysis, and the methods for using traditional architecture elements methods are summarized in an animation scene according to the feature of building shape in experiment pictures. The results show that the main visual resources of the viewers spend in animation character and that 1/5 resources are spent in building background. All spatial distance, layer relation and expression form of the building shape affect the recognition of the viewers. The application of traditional architecture elements in an animation scene design should emphatically express the structural feature and layer relation of the traditional buildings, focus on recreation of traditional architecture elements and the creation of the artistic conception, and choose the architecture elements according to the demand of animation contents.