In order to further analyze the biosynthetic pathway of dendrobine the literature review was conducted on the research progress of dendrobine biosynthesis pathway the function of CYP450 enzymes in biosynthesis of five medicinal terpenoids and the research methods of CYP450 enzymes. By summarizing the research progress of dendrobine biosynthesis the role of CYP450 enzyme in the hydroxylation intermediate of dendrobine biosynthesis was put forward. This paper summarized the research and identification of CYP450 enzyme functions in the synthetic pathways of medicinal terpenoids artemisinic acid tanshinone carnosic acid ginsenoside and glycyrrhizin and found that the main functions of CYP450 enzyme in terpenoids were oxidation functions such as hydroxylation and ketonization. This paper summarizes the research methods of CYP450 enzyme on one hand the screening of genes can be performed by database search or differential screening of plant tissues and multi-factor induced expression on the other hand the expression and function identification of genes are transferred into prokaryotic or eukaryotic host cells to express the enzyme protein through various means of genetic engineering and then the substrate is added to identify its catalytic activity. Finally looking forward to the next step in the mining of dendrobine biosynthetic CYP450 enzymes it is believed that the differential CYP450 enzyme genes can be screened from the existing transcriptome and protein expression and then the substrate can be obtained by upstream enzyme catalysis or separation and purification from plants and the CYP450 enzymes related to dendrobium alkaloids synthesis can be discovered by the catalysis of enzyme proteins and substrates.