Tenebrio molitor organic fertilizer (TMF) is a kind of high quality organic fertilizer. Existing studies have shown that it has positive effect on plant growth， but most of the evaluation indexes in literature only investigate the plant growth from simple indicators， relative indicators and composite indicators， and do not conduct in-depth study on the physical and chemical indicators in the process of plant growth. In order to solve this problem， five treatments (T1， T2， T3， T4， T5) were set up to test the changes of physicochemical index in the leaves of Pilea notata C. H. Wrigh， so as to investigate the effect of TMF on the growth and the optimal dosage of TMF. The results showed that the application of TMF had a great positive effect on the growth of Pilea notata C. H. Wrigh，especially outstanding in physicochemical index performance. The N content， chlorophyll content， POD activity， water content and plant height growth rate in T4 group were up to 106.96%， 59.47%， 75%， 5.60% and 30%， respectively, meanwhile, the effect of TMF in T4 group (9%)was the best.