At present, the features of all parts of China are weakening, the same feature in one thousand cities and cultural homogenization phenomena have emerged and regional culture homogenization phenomena are serious. According to the interior design of the hotels, the most representative design in interior design, by using concrete excavation and applied methods to integrate the regional culture into real space, the hotel operation situation is very good after completion of interior design, the customers recognition decree is high, the customer staying rate is higher than that of star hotels, which confirm the necessity of the research on regional culture. The highlighting of regional feature culture integration into the interior design of the hotels can provide cultural identification for human psychology, can bring freshness of different amorous feelings to the audiences in sense and mind, and by point and surface and gradually , can ultimately attain the goal of mitigating cultural homogenization and enhancing cultural self-confidence.