In the dynamic enterprises alliance, the interoperability of enterprises information systems is the foundation of enterprises collaboration. In order to solve this basic problem, which refers to interoperability of asynchronous systems between different enterprises, ontology thought was introduced, and Web Ontology Language (OWL) was used to establish ontology of mechanical accessory as well as Web Ontology LanguageService (OWLS) being used to establish web services. The ontology based integrated platform for interoperability of asynchronous systems was designed, meanwhile the semantic mapping rule for use in the process of ontology based interoperability of asynchronous systems was built. In the same way, the realization of interoperability of asynchronous systems was described. In the end a prototyping system was developed to validate the method. The results showed that using ontology technology could improve order dealing, also increase the performance of the platform in interoperability. Thus it provides an efficient approach to implement interoperability between asynchronous systems.