Discipline contest plays an important role in the integration of extracurricular and inclass practical teaching link and the innovation of talent cultivation mode for engineering courses. According to the present problem in the separation of discipline contest from practical teaching system and social demand, this paper puts forward practical innovation ability cultivation mode with the interaction of learning, contest and production for engineering talents, clarifies the significance and connotation for the interaction of learning, contest and production, based on practical exploration for the engineering major of Mechanical Engineering School of Chongqing Technology and Business University, studies and constructs discipline connotation system with the interaction of learning, contest and production from such three aspects as the integration of practical link, the optimization of theoretical teaching contents and building enterprise practice teaching platform, and provides certain reference for the talent cultivation majoring in manufacture.