Because crude bamboo vinegar is a kind of very complex mixture and is difficult to be widely used, the complex crude bamboo vinegar must be refined in order to better take advantage of its functions. The crude bamboo vinegar is processed by ozone oxidation method in the way of the combination of air and liquid by mixture pump and Millipore diffuser. The influence of oxidation time, oxidation method and ozone aerating amount on physical and chemical property in the bamboo vinegar is discussed. The conclusion is that the refined bamboo vinegar disease its optical absorbency, pH value and tar content but increases its total acidity and acetic acid content under the optimal condition of Millipore aeration, 7 h oxidation and 23.76 g/h aerating ozone amount.