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| 摘要: |
| 介绍了实时定量RT-PCR的原理、方法和应用,包括SYBR Green I、AmpliSensor、Lightcycle技术和Complex探针技术;同时还对原位PCR技术的原理、方法和应用,简并PCR的原理、方法和应用作了综述。 |
| 关键词: PCR,实时定量RT-PCR,原位PCR,简并PCR |
| DOI： |
| 分类号:Q819 |
| 基金项目: |
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| New progress of polymerase chain reaction technology |
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JIANG Huai-chun~1 LI Hong~2
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| Abstract: |
| Polymerase chain reaction technology has developed into a classic experimental method in molecular biology and genetics field and so on. This paper introduced the principle, method and application of Real Time Quantitative RT-PCR which includes SYBR Green |
| Key words: polymerase chain reaction,Real Time Quantitative RT-PCR,In Situ PCR,Degenerate PCR |
