The dissipative evolution of the innovation ecosystem in the high-tech industry can promote innovation-driven high-quality development and alleviate the problem of unbalanced regional innovation development. The Brusselator model is interpreted in an economic way, and the evaluation index system of “innovation input-innovation environment” framework is constructed, which can deduce the judgment conditions of the formation of the dissipative structure of the innovation ecosystem of high-tech industry, and describe the process of the system dissipation evolution by using the “global entropy-catastrophe series” evaluation model. Based on the data of 30 provincial high-tech industries in China from 2013 to 2019, the analysis shows that the high-tech industry innovation ecosystem in Beijing, Tianjin and Shanghai has formed a dissipative structure, and most of the regions have not formed a dissipative structure, but are evolving toward the dissipative structure. The two-dimensional framework of “innovation input-innovation environment” of the high-tech industry innovation ecosystem in the sample areas presents an overall evolution trend of “Quadrant Ⅲ → Quadrant Ⅱ → Quadrant Ⅰ”, and the evaluation value of “innovation input-innovation environment” tends to increase, and different regions have different characteristics of dissipative evolution. While increasing investment in high-tech industry innovation, it is necessary to devote ourselves to improving the innovation environment, and actively build a collaborative innovation network of innovation subjects. In order to further promote the dissipative evolution of the high-tech industry innovation ecosystem, each region should not only adopt differentiated development strategies, but also promote the coordinated development among regions.