The entrepreneurial opportunity identification is the important premise for entrepreneurship realization and is also important guarantee for entrepreneurship success. By using questionnaire survey data, inspection of the Three Gorges reservoir immigrants entrepreneurs finds that first previous experience and information resources of immigrant entrepreneurs (previous entrepreneurial experience, previous management skills training experience and previous entrepreneurship training experience） have significantly positive influence on entrepreneurial opportunity recognition. Second, the information resources in a previous entrepreneurial experience play completely intermediary role between entrepreneurship training experience and entrepreneurial opportunity identification, information resources in the previous entrepreneurship training experience play partial intermediary role between entrepreneurial opportunity identification. Third, the policy environment negatively regulates the relationship between the information resources of immigrant entrepreneurs and the recognition of entrepreneurial opportunities. The improvement of policy environment and making the entrepreneurs accept more information from normal channels make importance decrease for the information through abnormal channels. Therefore, good policy environment is conducive to more entrepreneurs to identify entrepreneurial opportunity and entrepreneurship realization.