In a perspective of spatial economics, the impact of the service industry agglomeration on regional economic growth is studied by physical capital agglomeration, human capital agglomeration, employment density and spatial econometric methods, the results show that the regional economic growth of China demonstrates the obvious characteristic of spatial agglomeration, that the propelling action of service industry physical capital agglomeration level on regional economic development presents weakening trends, that the effect of human capital agglomeration on regional economic growth changes from positive to negative, however, service industry employment density, economic opening level and so on play more and more important role. China should emphatically consolidate the exchange and cooperation between neighboring regions for supporting each other in economy and coordinated development, meanwhile, promote China’s service industry agglomeration and sufficiently let service industry boost the positive action on regional economic growth.