As the main part of innovation activities, scientific and technological enterprises are facing the problem of financing difficulties because of the typical characteristics of "light assets" caused by the capitalization of intellectual property rights. Therefore, in 2017, Chongqing officially launched the pilot reform of knowledge value credit loan for science and technology-based enterprises, carried out knowledge value credit evaluation for science and technology-based enterprises, and implemented pure credit loan by cooperative banks, which initially explored to solve the financing problem of science and technology-based enterprises. In 2018, Chongqing Science and Technology Bureau optimized the evaluation model of scientific and technological knowledge value, and gradually expanded the scope of the pilot. This paper comprehensively analyzes the general idea and process of the knowledge value credit loan of Chongqing science and technology-based enterprises in 2018. Through the empirical analysis of the knowledge value financing of Chongqing science and technology-based enterprises, it summarizes and puts forward the ways to solve the problem by innovating the government's policy support, strengthening the construction of the credit system of science and technology-based enterprises, improving the bank's financial innovation service, and reinforcing the multi-sector cooperation.