The photosynthetic bacteria PSBZF1，identified as Rhodospirillum by bacteria dyeing，physiological and biochemical test and absorption spectrum test of live cells，was acquired in porcine dung by means of flat lining separation. Through experiment analysis of its nutrition condition in the process of cultivation，the best composition of culture medium was received as follows: sodium acetate 2.0g，ammonium chloride 1.0g，sodium bicarbonate 0.5g，sodium chloride 1.0g，potassium dihydrogen phosphate 0.2g，magnesium chloride hexahydrate 0.2g，T.M storage solution 1.0mL，yeast cream 0.8g，and distilled water 1000mL. By examining the influence of environment factors on the bacteria growth，the best growth condition for PSBZF1 was obtained as follows: inoculums of 10%，illumination intension of 3000lux，dissolved oxygen 2.02mg/L，initial pH 7，and under stirring condition. High concentration of PSBZF1 culture is conducive to continuous hydrogen production experiment.